| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Thermosipho melanesiensis | TTTTGGTGGGGTAGGCCAC | GCTGTACGCTCCTGAAAGC | 59.85 | 58.92 | 295 | 147 |
100.00%
|
100.00%
|
| Primer 2 | Thermosipho melanesiensis | GCGACAAACTATGAGGGAAGT | TGCCGTTAAATTGCCTCCAT | 58.03 | 58.15 | 225 | 143 |
100.00%
|
100.00%
|
| Primer 3 | Thermosipho melanesiensis | CACACGTGGAGGAACAATCC | AGTTTGTTGTATGGTGCGGAT | 58.84 | 58.21 | 237 | 141 |
100.00%
|
100.00%
|
| Primer 4 | Thermosipho melanesiensis | ACACACGTGGAGGAACAATCC | AGTTTGTTGTATGGTGCGGAT | 60.54 | 58.21 | 238 | 141 |
100.00%
|
100.00%
|
| Primer 5 | Thermosipho melanesiensis | GACACACGTGGAGGAACAATC | AGTTTGTTGTATGGTGCGGAT | 59.20 | 58.21 | 239 | 141 |
100.00%
|
100.00%
|
| Primer 6 | Thermosipho melanesiensis | ACACACGTGGAGGAACAATC | AGTTTGTTGTATGGTGCGGAT | 58.12 | 58.21 | 238 | 141 |
100.00%
|
100.00%
|
| Primer 7 | Thermosipho melanesiensis | ATTGGTGGAGGCGATGGTG | TGACCACCTTCACCTGCAG | 60.08 | 59.55 | 254 | 140 |
100.00%
|
100.00%
|
| Primer 8 | Thermosipho melanesiensis | TGCACCAGAAGCAAGAGGC | AAAGCAAGTGCAGTTCCCC | 60.60 | 58.57 | 231 | 140 |
100.00%
|
100.00%
|
| Primer 9 | Thermosipho melanesiensis | TGGGGAATTAGGAAAACGGCA | TCACTCTTCCACCTTCTCGGA | 59.92 | 60.20 | 231 | 138 |
100.00%
|
100.00%
|
| Primer 10 | Thermosipho melanesiensis | TGCAGCTGAAATAAGTGCCC | TGCGAATTTTCTGCTTCGACT | 58.82 | 58.86 | 192 | 138 |
100.00%
|
100.00%
|